LCK, GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Enzyme reaction is conducted at 30°C for 1 hour in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate using Z-Lyte assay conditions.

Description: Recombinant human LCK, GenBank Accession No. NM_005356, full length, with N-terminal GST-tag, MW = 83 kDa, expressed in Sf9 insect cells using a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT

Genbank: NM_005356

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: P06239

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Fischer, S. et al. Biochem Biophys Res Commun. 1987 Mar 30,143(3):819-26.
2. Veillette, A. et al. Oncogene Res. 1987 Sep-Oct,1(4):357-74.

• Artikelnummer: BPS40470
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40470
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect