English
LDHA, His-tag Recombinant
LDHA, His-tag Recombinant
Artikelnummer
BPS71081
Verpackungseinheit
100 µg
Hersteller
BPS Bioscience
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 2-332(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was conducted in 100 mM Tris, pH 7.4, 2 mM sodium pyruvate, 0.2 mM β-NADH, 0.02% BSA, and LDHA in a volume of 200 µL. Initiate reactions with LDHA. Monitor the decrease in NADH absorbance at 340 nm at 37°C for 5 min. Read at Abs340 using Tecan Infinite 1000.
Description: Human lactate dehydrogenase A (LDHA), GenBank Accession No. NM_005566), full length [2-332(end)] with N-terminal His-tag, MW= 37.5kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 160 mMimidazole, 0.04% Tween-20, and 20%glycerol.
Genbank: NM_005566
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P00338
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rong Y., et al. Tumour Biol. 2013. 34(3):1523-30.
2. Tang H., et al. Biol Reprod. 2013. 88(4):96.
Encompassing Amino Acids: 2-332(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was conducted in 100 mM Tris, pH 7.4, 2 mM sodium pyruvate, 0.2 mM β-NADH, 0.02% BSA, and LDHA in a volume of 200 µL. Initiate reactions with LDHA. Monitor the decrease in NADH absorbance at 340 nm at 37°C for 5 min. Read at Abs340 using Tecan Infinite 1000.
Description: Human lactate dehydrogenase A (LDHA), GenBank Accession No. NM_005566), full length [2-332(end)] with N-terminal His-tag, MW= 37.5kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 160 mMimidazole, 0.04% Tween-20, and 20%glycerol.
Genbank: NM_005566
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P00338
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rong Y., et al. Tumour Biol. 2013. 34(3):1523-30.
2. Tang H., et al. Biol Reprod. 2013. 88(4):96.
| Artikelnummer | BPS71081 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 71081 |
| Green Labware | Nein |
| Verpackungseinheit | 100 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
