LKB1/MO25/STRAD Complex Recombinant

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Encompassing Amino Acids: LKB1 2-433, MO25 2-341, STRAD 2-431

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 100 µM ATP and 0.2 mg/ml LKBtide peptide was used as a substrate. Reaction was incubated at 30°C for 30 min. Reaction progress was monitored using ADP-Glo reagents (Promega).

Description: Complex of human LKB1, also known as STK11 (GenBank Accession No. NM_000455, a.a. 2-433, with N-terminal His-tag), MO25 (GenBank Accession No. NM_016289, a.a. 2-341, with N-terminal GST-tag), and STRAD (GenBank Accession No. NM_001003787, a.a. 2-431, with N-terminal GST-tag). Calculated MW=49 kDa* (LKB1), 66 kDa (MO25), and 74 kDa (STRAD), expressed in Sf9 cells using a Baculovirus expression system. *LKB1 runs anomalously on SDS-PAGE.

Format: Aqueous buffer solution

Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol

Genbank: STK11 (NM_000455), MO25 (NM_016289), STRAD (NM_001003787)

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: STK11, N-terminal His-tag, MO25, N-terminal GST-tag, STRAD, N-terminal GST-tag

Uniprot: LKB1: Q15831; MO25: Q9Y376; STRAD: Q7RTN6

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Yoo, L.I., et al. (2002). Nat. Rev. Cancer 2(7): 529-535.
2. Katajisto P, et al. (2007). Biochim. Biophys. Acta 1775 (1): 63-75.

• Artikelnummer: BPS40069
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40069
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect