LSD1 (KDM1A), Variant 2, GST-tag Recombinant

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Encompassing Amino Acids: 158-end

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 µl reaction mix containing assay buffer with 50 mM Tris HCl pH 8.8, 50 mM NaCl, 1mM DTT, 0.01% Tween-20, histone H3 peptide substrate, and LSD1 (6.7 - 40 ng) add to the wells. Incubate for 1 hr. Add antibody against unmethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Description: Human LSD1 [also known as KDM1A (lysine (K)-specific demethylase 1A) or AOF2, amine oxidase (flavin containing) domain 2)] GenBank Accession No. NM_015013, a.a. 158-end with N-terminal GST-tag, MW=103 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Genbank: NM_015013

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: O60341

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Lan,F. et al., Nature 448, 7154, 718-722, 2007.
2. Forneris,F. et al., J. Biol. Chem. 282, 28, 20070-20074, 2007.Application Reference(s):
1. A scintillation proximity assay for histone demethylases (2014)
2. Crystal structure of the histone lysine specific demethylase LSD1 complexed with tetrahydrofolate (2014)

• Artikelnummer: BPS50100
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50100
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK