LSD2 (KDM1B), GST-Tev-Tag Recombinant

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Encompassing Amino Acids: 2-822

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 µL of reaction mixture contains 50 mM Tris, pH 8.8, 50 mM NaCl, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and various amount of LSD2. The reactions were incubated at room temperature for one hour with slow shaking. After the reaction, 10 µL of primary antibody (1:800 dilution) and anti-mouse acceptor beads (1:500 dilution) was added into the wells and the reactions were incubated for 30 min. Then 10 µL of Streptavidin-conjugated donor beads (1:125 dilution) was added into the wells. The reactions were incubated for additional 30 min at room temperature followed by Alpha screening detection.

Description: Human LSD2 protein, also known as KDM1B, GenBank Accession No. NM_153042, a.a. 2-822 with N-terminal GST-Tev-Tag. MW = 121 kDa, expressed in Sf9 cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and 1.6 mM glutathione

Genbank: NM_153042

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Tags: N-terminal GST-Tev Tag

Uniprot: Q8NB78

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1.Binda, C., et al., Journal of American Chemical Society. 2010; 132(19): 6827-6833.2.Welte, M., et al., Cell Press. 2005; 15(14): 1266-1275.

• Artikelnummer: BPS50124
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50124
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect