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Encompassing Amino Acids: 1-611 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human leukotriene A-4 hydrolase, LTA4H, (GenBank Accession No. NM_000895, a.a. 1-611(end) with C-terminal FLAG-tag and His-tag, MW = 71 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 100 mM imidazole, and 20% glycerol.
Genbank: NM_000895
Purification: Protein was purified by affinity chromatography.
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-His-tags
Uniprot: P09960
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Snelgrove, R.J., et al., Science 2010: 330(2000):90-94.
2. Stsiapanava, A., et al., PNAS 2014: 111(11):4227-4232