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Encompassing Amino Acids: 637-926
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: The specific activity was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme for 5 minutes at 30°C.
Background: PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. This PTP acts at junctions between the membrane and the cytoskeleton and has been shown to interact with glutamate receptor delta 2 and epsilon subunits. It also is thought to play a role in signalling downstream of the glutamate receptors through tyrosine dephosphorylation.
Description: MEG-1 protein tyrosine phosphatase (PTP-MEG-1), Genbank Accession No. NM_002830, a.a. 637-926 (catalytic domain) with N-terminal GST-tag, MW=60.3 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_002830
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: P29074
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. K. Hironaka, et al., J. Biol.Chem. 2000, 275: 16167-16173
2. M. Gu, et al. Proc. Natl. Acad. Sci. 1996, 93: 12980-12985