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Encompassing Amino Acids: 2-580
Applications: Useful as a positive control for Western blot studies.
Description: Human Methyltransferase-like 3 (METTL3), GenBank Accession No. NM_019852, 2-580 (end) with N-terminal FLAG-tag, expressed by baculovirus in Sf9 insect cells. MW = 66 kDa.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, 100 µg/ml FLAG peptide
Genbank: NM_019852
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-Tag
Uniprot: Q86U44
Warnings: Avoid freeze/thaw cycles. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Biosafety Level: Not applicable (BSL-1)
References: 1. Liu J. et al. Nat. Chem. Biol. 2014. 10: 93-952. Wang X. et al. Nature. 2016. 534:575578