Mini Sample Mouse SAA ( Serum Amyloid A ) ELISA Kit

Mini Sample Mouse SAA ( Serum Amyloid A ) ELISA Kit
Artikelnummer
ELSE-MSEL-M0091-48
Verpackungseinheit
48 T
Hersteller
Elabscience Biotechnology

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Storage: 2-8℃/-20℃,12 months

Abbreviation: SAA

Target Synonym: PIG4,SAA,SAA1,Serum amyloid A1,TP53I4

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse SAA concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, plasma and other biological fluids

Type: Sandwich-ELISA

Assay Time: 3 h 30 min

Detection Type: Colorimetric method;ELISA;Sandwich

Sensitivity: 0.01ng/mL

Detection Range: 0.16-10 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SAA . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SAA , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of SAA . You can calculate the concentration of SAA in the samples by comparing the OD of the samples to the standard curve.

Cross_reactivity: This kit recognizes SAA in samples.No significant cross-reactivity or interference between SAA and analogues was observed.
Mehr Informationen
Artikelnummer ELSE-MSEL-M0091-48
Hersteller Elabscience Biotechnology
Hersteller Artikelnummer E-MSEL-M0091-48
Verpackungseinheit 48 T
Mengeneinheit PAK
Methode ELISA
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