MLCK, GST-tag Recombinant

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Encompassing Amino Acids: 1425-1776

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: MLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and is a calcium/calmodulin dependent protein kinase. MLCK is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal (1). Phosphorylation of regulatory light chain of myosin plays an important role in controlling the morphological changes seen during cell division (2).

Description: Recombinant human MLCK (a.a. 1425-1776), with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_053025

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q15746

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Gao, Y. et al: Myosin light chain kinase as a multifunctional regulatory protein of smooth muscle contraction. IUBMB Life. 2001 Jun;51(6):337-44.
2. Matsumura, F. et al: Role of myosin light chain phosphorylation in the regulation of cytokinesis. Cell Struct Funct. 2001 Dec;26(6):639-44.

• Artikelnummer: BPS40127
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40127
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect