MLK1, GST-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 1-433

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Varying concentrations of MLK1 were mixed with 5 µg of MBP protein substrate and 5 µl of 250 µM [³³P]-ATP was added for a total volume of 25 µl. Mixture was incubated for 15 min at 30°C. Reaction was stopped by placing 20 µl of reaction onto phosphocellulose P81 paper. Paper was washed 3 times with 1% phosphoric acid solution for 10 min each and air dried. Radioactivity was measured from the P81 paper in the presence of scintillation fluid using a scintillation counter.

Description: Human MLK1, also known as Mixed Lineage Kinase 1, Mitogen-activated protein kinase kinase kinase 9, MAP3K9, MEKK9, and PRKE1, GenBank Accession No. NM_033141, a.a. 1-433, with N-terminal GST-tag, MW = 77 kDa, expressed in Sf9 cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, ph 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_033141

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: P80192

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Dorrow, D.S., et al., Europ. J. Biochem. 1993. 213:701-710.
2. Durkin, J.T., et al., Biochemistry. 2004. 43(51):16348-16355.

• Artikelnummer: BPS41102
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 41102
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect