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MMP2, His-tag (HEK293-derived) Recombinant
MMP2, His-tag (HEK293-derived) Recombinant
Artikelnummer
BPS80213
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 30 - 660 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Activation: This is a proform enzyme andrequires activation prior to testing activity.Dilute the enzyme to 100 ng/ml in a solutioncontaining 50 mM HEPES, pH 7.4, 10 mMCaCl2, 0.05% Brij-35, and 1 mM APMA(amino-phenyl mercuric acetate). Incubate at37°C for 2 hours.
Assay conditions: Reaction mixture with 10 µM390 MMP substrate 1 (Anaspec). Incubate for30 minutes at room temperature. Fluorescenceintensity is measured at exc328/em393.
Description: Human matrix metallo-peptidase-2 (MMP2), also known as gelatinase A (GenBank Accession No. NM_004530), a.a. 30 - 660 (end), with C-terminal His-tag, MW=72 kDa, expressed in an HEK293 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Genbank: NM_004530
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P08253
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Van Dijk, M., et al.,Front Genet.2012; 3:22.2. Klotzsche-von Ameln, A., et al.,Cancer Biol Ther. 2012 Feb 15;13(4): 216 - 223.
Encompassing Amino Acids: 30 - 660 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Activation: This is a proform enzyme andrequires activation prior to testing activity.Dilute the enzyme to 100 ng/ml in a solutioncontaining 50 mM HEPES, pH 7.4, 10 mMCaCl2, 0.05% Brij-35, and 1 mM APMA(amino-phenyl mercuric acetate). Incubate at37°C for 2 hours.
Assay conditions: Reaction mixture with 10 µM390 MMP substrate 1 (Anaspec). Incubate for30 minutes at room temperature. Fluorescenceintensity is measured at exc328/em393.
Description: Human matrix metallo-peptidase-2 (MMP2), also known as gelatinase A (GenBank Accession No. NM_004530), a.a. 30 - 660 (end), with C-terminal His-tag, MW=72 kDa, expressed in an HEK293 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Genbank: NM_004530
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P08253
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Van Dijk, M., et al.,Front Genet.2012; 3:22.2. Klotzsche-von Ameln, A., et al.,Cancer Biol Ther. 2012 Feb 15;13(4): 216 - 223.
| Artikelnummer | BPS80213 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 80213 |
| Green Labware | Nein |
| Verpackungseinheit | 20 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
