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MMP9 (Q279R), His-tag Recombinant
MMP9 (Q279R), His-tag Recombinant
Artikelnummer
BPS80215
Verpackungseinheit
20 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 20-707 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Activation: This is a proform enzyme andrequires activation prior to testing activity. Dilutethe enzyme to 100 ng/ml in a solution containing50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05%Brij-35, and 1 mM APMA (amino-phenylmercuric acetate). Incubate at 37°C for 2 hours.
Assay conditions: Reaction mixture with 10 µM390 MMP substrate 1 (Anaspec). Incubate for30 minutes at room temperature. Fluorescenceintensity is measured at exc328/em393.
Description: Human matrix metallopeptidase-9 (MMP9), also known as gelatinase B, MANDP2, CLG4B, Macrophage gelatinase, GELB and EC 3.4.24, with Gln to Arg mutation on a.a. 279, GenBank Accession No. NM_004994), a.a. 20-707 (end) with C-terminal His-tag, MW=77.8 kDa, expressed in an HEK293 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Genbank: NM_004994
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P14780
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wenham, R.M., et al., J. Soc. Gynecol.Investig. 2003 Sep;10(6):381-7.
2. Li, X., et al., J. Cancer Res. Clin. Oncol.2013 Sep;139(9):1433-47.
Encompassing Amino Acids: 20-707 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Activation: This is a proform enzyme andrequires activation prior to testing activity. Dilutethe enzyme to 100 ng/ml in a solution containing50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05%Brij-35, and 1 mM APMA (amino-phenylmercuric acetate). Incubate at 37°C for 2 hours.
Assay conditions: Reaction mixture with 10 µM390 MMP substrate 1 (Anaspec). Incubate for30 minutes at room temperature. Fluorescenceintensity is measured at exc328/em393.
Description: Human matrix metallopeptidase-9 (MMP9), also known as gelatinase B, MANDP2, CLG4B, Macrophage gelatinase, GELB and EC 3.4.24, with Gln to Arg mutation on a.a. 279, GenBank Accession No. NM_004994), a.a. 20-707 (end) with C-terminal His-tag, MW=77.8 kDa, expressed in an HEK293 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Genbank: NM_004994
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P14780
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wenham, R.M., et al., J. Soc. Gynecol.Investig. 2003 Sep;10(6):381-7.
2. Li, X., et al., J. Cancer Res. Clin. Oncol.2013 Sep;139(9):1433-47.
| Artikelnummer | BPS80215 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 80215 |
| Green Labware | Nein |
| Verpackungseinheit | 20 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
