Mouse anti-AHNAK1 Purified, clone EM-09 (Monoclonal)

Clone Number: EM-09

Immunogen: Bacterially expressed fragment of N-terminal domain of human AHNAK1.

Concentration: 1 mg/ml

Format: Purified by protein-A affinity chromatography.

Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Additional info: AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.SpecificityThe antibody TU-08 recognizes alpha, beta-tubulin heterodimer (porcine brain), a basic intracellular structural unit of microtubules. Alpha- and beta- tubulins form approximately 100 kDa tubulin heterodimer, a globular protein that polymerizes to form microtubules.Application detailsImmunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required.
Western blotting: Positive control: HeLa human cervix carcinoma cell line.

• Artikelnummer: SANMON23009
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23009
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine)
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Immunoprecipitation, Western Blotting, Immunocytochemistry
• Isotyp: IgG1
• Wirt: Mouse
• Konjugat: Unconjugated