Mouse anti-Clathrin Heavy Chain Purified, clone BF-06 (Monoclonal)

Clone Number: BF-06

Immunogen: Unknown or proprietery to MONOSAN and/or its suppliers

Concentration: 1 mg/ml

Format: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Storage buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Additional info: Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.SpecificityMouse monoclonal antibody CLIC5-02 recognizes CLIC5a, a 32 kDa intracellular protein which associates with proteins of actin complexes. Crossreactivity with CLIC5b was not determined.Application detailsFlow cytometry: Recommended dilution: 2-5 μg/ml; positive control: human blood leukocytes. Intracellular staining.

• Artikelnummer: SANMON23028
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23028
• Green Labware: Nein
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus), Pig (Porcine), Cow (Bovine)
• Klonalität: Monoclonal
• Methode: Immunoprecipitation, Western Blotting, ELISA, Flow Cytometry, Immunocytochemistry
• Isotyp: IgM
• Wirt: Mouse