Mouse anti-Clostridium dificile Toxin A, clone EBS-I-100 (Monoclonal)

Clone Number: EBS-I-100

Immunogen: C.difficile toxin A

Concentration: 100 ug/ml

Storage buffer: PBS with 0.02% sodium azide

Additional info: EBS-I-100 reacts with C. difficile Toxin A, but not with V. cholerae subunit a, V. cholerae toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. C. difficile is a major nosocomial pathogen that causes antibiotic-associated colitis and mediates inflammatory diarrhea by releasing two large protein enterotoxins (toxin A and toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. C. difficile toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. C. difficile toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.

References: Kim H, et al, Gastroenterology 129: 1875-1888 (2005)/Carter JP, et al, Gut Microbes. 1(1): 58–64 (2010)

• Artikelnummer: SANMON9883
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON9883
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Various species
• Klonalität: Monoclonal
• Methode: Immunofluorescence, Immunohistochemistry (frozen), ELISA
• Isotyp: IgG3-K
• Wirt: Mouse
• Konjugat: Unconjugated