Mouse anti-Galectin-3, clone B2C10 (Monoclonal)

Clone Number: B2C10

Immunogen: Unknown or proprietery to MONOSAN and/or its suppliers

Concentration: 100 ug/ ml

Storage buffer: PBS with 0.1% BSA and 0.02% sodium azide

Additional info: The monoclonal antibody B2C10 reacts with galectin-3, a 30 kDa protein. Galectin-3 is a member of the galectin family. The protein is composed of three domains: a small amino-terminal domain, a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma, while down-regulated in other types of malignancies, such as colon, breast, ovarian and uterine carcinomas.
Galectin-3 has been shown to function through both intracellular and extracellular actions. Related to its intracellular functions, galectin-3 has been identified as a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing, and has been found to control cell cycle and prevent T cell apoptosis. On the other hand, this protein has also been demonstrated to function as extracellular molecule in activating various types of cells, including monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions.
The monoclonal antibody B2C10 inhibits the binding of 125I-labeled galectin-3 to IgE coated on microtiter plates, the galecin-3’s hemagglutination activity and galectin-3-induced superoxide production by human neutrophils. This inhibitory activity of B2C10 is probably the result of its disruption of the self-association process.
The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of galectin-3. The antibody B2C10 does not react with Galecin-3C and is cross reactive with mouse galectin-3.

References: Liu; F et al. Biochemistry 1996; 35: 6073 /Sano, H et al J Immunol 2000, 165: 2156 /Feilchenfeldt; J et al. Mod Pathol 2003; 16: 1117

• Artikelnummer: SANMON7075
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON7075
• Green Labware: Nein
• Verpackungseinheit: 1 ml
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine)
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western Blotting, ELISA, Flow Cytometry, Functional Assay
• Isotyp: IgG1
• Wirt: Mouse