Mouse anti-Hu CD146 Purified, clone P1H12 (Monoclonal)

Clone Number: P1H12

Immunogen: cultured human umbilical cells

Concentration: 1 mg/ml

Format: Purified by protein-A affinity chromatography.

Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Additional info: CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50% of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.SpecificityThe antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.Application detailsWestern blotting: Nonreducing conditions.
Flow cytometry: Recommended dilution: 1-4 µg/ml

• Artikelnummer: SANMON23095
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23095
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rabbit, Dog (Canine)
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Immunoprecipitation, Western Blotting, Flow Cytometry, Immunocytochemistry
• Isotyp: IgG1
• Wirt: Mouse
• Konjugat: Unconjugated