Mouse anti-Hu CD15 Purified, clone MMA (Monoclonal)

Clone Number: MMA

Immunogen: U937 histiocytic lymphoma cells

Concentration: 1 mg/ml

Format: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Storage buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Additional info: CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.SpecificityThe mouse monoclonal antibody CD151 recognizes an extracellular epitope of CD151 (also known as PETA-3), a 29 kDa transmembrane protein of tetraspanin family, expressed in many cell types.Application detailsFlow cytometry: Recommended dilution: 1-4 μg/ml.

• Artikelnummer: SANMON23098
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23098
• Green Labware: Nein
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Flow Cytometry, Immunocytochemistry
• Isotyp: IgM kappa
• Wirt: Mouse