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Mouse anti-Hu CD18 Purified, clone MEM-48 (Monoclonal)
Mouse anti-Hu CD18 Purified, clone MEM-48 (Monoclonal)
Artikelnummer
SANMON23122
Verpackungseinheit
0,1 mg
Hersteller
Sanbio / Monosan
Verfügbarkeit:
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Clone Number: MEM-48
Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.
Concentration: 1 mg/ml
Format: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.SpecificityThe mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.Application detailsWestern blotting: Recommended dilution: 2 μg/ml; positive control: Kg-1a human leukemia cell lysate, non-reducing conditions.
Flow cytometry: Recommended dilution: 2 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen, microglia.
Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.
Concentration: 1 mg/ml
Format: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.SpecificityThe mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.Application detailsWestern blotting: Recommended dilution: 2 μg/ml; positive control: Kg-1a human leukemia cell lysate, non-reducing conditions.
Flow cytometry: Recommended dilution: 2 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen, microglia.
| Artikelnummer | SANMON23122 |
|---|---|
| Hersteller | Sanbio / Monosan |
| Hersteller Artikelnummer | MON23122 |
| Green Labware | Nein |
| Verpackungseinheit | 0,1 mg |
| Mengeneinheit | STK |
| Reaktivität | Human |
| Klonalität | Monoclonal |
| Methode | Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Immunoprecipitation, Western Blotting, Flow Cytometry |
| Isotyp | IgG1 |
| Wirt | Mouse |
| Produktinformation (PDF) |
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| MSDS (PDF) |
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Gernot Ensinger, M.Sc.
Laurent Haze
