Mouse anti-Hu CD64 Purified, clone 10.1 (Monoclonal)

Clone Number: 10,1

Immunogen: Rheumatoid synovial fluid cells and fibronectin purified human monocytes

Concentration: 1 mg/ml

Format: Purified by protein-A affinity chromatography.

Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Additional info: CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.SpecificityThe mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly).Application detailsFlow cytometry: Recommended dilution: 1-5 μg/ml.

• Artikelnummer: SANMON23213
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23213
• Green Labware: Nein
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Primate
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Immunoprecipitation, Western Blotting, Flow Cytometry, Immunocytochemistry
• Isotyp: IgG1 kappa
• Wirt: Mouse