Mouse anti-Hu CD8 Purified, clone MEM-87 (Monoclonal)

Clone Number: MEM-87

Immunogen: Leukocytes of a patient suffering from LGL Type Leukemia.

Concentration: 1 mg/ml

Format: Purified by protein-A affinity chromatography.

Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Additional info: The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.SpecificityThe antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.Application detailsFlow cytometry: Recommended dilution: 2 μg/ml.
Immunoprecipitation: Excellent for immunoisolation of CD8 positive T cells.

• Artikelnummer: SANMON23229
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23229
• Green Labware: Nein
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Immunoprecipitation, Flow Cytometry
• Isotyp: IgG1
• Wirt: Mouse