Mouse anti-Hu STRO-1 Purified, clone STRO-1 (Monoclonal)

Clone Number: STRO-1

Immunogen: Human CD34 positive bone marrow cells

Concentration: 1 mg/ml

Format: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Storage buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Additional info: STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.SpecificityThe mouse monoclonal antibody W5C5 recognizes an extracellular epitope of SUSD2, a type I transmembrane protein expressed on mesenchymal stem-like cells. This antibody selectively binds to a MSCs in both bone marrow and endometrium or tonsil, and can be used for their identification and isolation.Application detailsFlow cytometry: Recommended dilution: 1-5 μg/ml.

• Artikelnummer: SANMON23265
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON23265
• Green Labware: Nein
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Rat (Rattus), Rabbit
• Klonalität: Monoclonal
• Methode: Flow Cytometry, Immunocytochemistry
• Isotyp: IgM lambda
• Wirt: Mouse