Mouse anti Human Caspase-9

Mouse anti Human Caspase-9, Monoclonal, IgG, Clone: LAAP6 96 2-22

Clone: LAAP6 96 2-22.

Background: Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-9 is a member of the CED-3 family and bear high similarity to caspase-3. Procaspase-9 can be activated by either caspase-3 or granzyme B, although they cleave the proenzyme to different size subunits. Cleavage by granzyme B produces an active enzyme which is capable of cleaving PARP. Also, the ability of caspase-3 to activate caspase-9 seems to suggest that caspase-9 is further downstream of caspase-3 and may be involved in later changes in cells observed undergoing apoptosis.

Positive Control: MCF-7 cell lines.

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-9 protein and mouse myeloma cells.

Purification Method: Protein A/G Chromatography.

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.

References: 1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16

2. Stennicke, H.R., et al. Caspase-9 can be activated without proteolytic processing. J. Biol. Chem. 1999, 274, 8359-8362

3. Kuida, K. Caspase-9. Int. J. Biochem. Cell Biol. 2000, 32, 121-124.

UniProt: P55211.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

• Artikelnummer: EXAX1177M
• Hersteller: Exalpha Biologicals Inc
• Hersteller Artikelnummer: X1177M
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Western Blotting
• Isotyp: IgG
• Wirt: Mouse