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Mouse anti Human p34 cdc-2 nuclear protein
Mouse anti Human p34 cdc-2 nuclear protein, Monoclonal, IgG2a, Clone: POH-1
Artikelnummer
EXAX1236M
Verpackungseinheit
100 µg
Hersteller
Exalpha Biologicals Inc
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Clone: POH-1.
Background: p34(Cdc2) kinase is a an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.
Positive Control: HeLa cell lysate.
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.
Purification Method: Protein A/G Chromatography.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Lukas, J., et al. Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur. J. Biochem. 1992, 207, 169-176
2. Borgne, A. & Meijer, L. Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition. J. Biol. Chem. 1996, 271, 27847-27854
3. Kao, G.D., et al. p34(Cdc2) kinase activity is excluded from the nucleus during the radiation-induced G2 arrest in HeLa cells. J. Biol. Chem. 1999, 274, 34779-34784.
UniProt: P06493.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Background: p34(Cdc2) kinase is a an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.
Positive Control: HeLa cell lysate.
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.
Purification Method: Protein A/G Chromatography.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Lukas, J., et al. Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur. J. Biochem. 1992, 207, 169-176
2. Borgne, A. & Meijer, L. Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition. J. Biol. Chem. 1996, 271, 27847-27854
3. Kao, G.D., et al. p34(Cdc2) kinase activity is excluded from the nucleus during the radiation-induced G2 arrest in HeLa cells. J. Biol. Chem. 1999, 274, 34779-34784.
UniProt: P06493.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
| Artikelnummer | EXAX1236M |
|---|---|
| Hersteller | Exalpha Biologicals Inc |
| Hersteller Artikelnummer | X1236M |
| Green Labware | Nein |
| Verpackungseinheit | 100 µg |
| Mengeneinheit | STK |
| Reaktivität | Human, Mouse (Murine), Rat (Rattus), Cow (Bovine), Frog (Xenopus Laevis) |
| Klonalität | Monoclonal |
| Methode | Immunohistochemistry (paraffin), Immunoprecipitation, Western Blotting, Immunocytochemistry |
| Isotyp | IgG2a |
| Wirt | Mouse |
| Produktinformation (PDF) | Download |
| MSDS (PDF) | Download |
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Gernot Ensinger, M.Sc.
