Mouse anti-VCP (Intra-acrosomal protein), clone Hs-14 (Monoclonal)

Clone Number: Hs-14

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Concentration: 1 mg/ml

Storage buffer: Tris buffered saline (TBS) solution with 15 mM sodium azide

Additional info: VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

• Artikelnummer: SANMON8017
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON8017
• Green Labware: Nein
• Verpackungseinheit: 0,1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine)
• Klonalität: Monoclonal
• Methode: Western Blotting, Flow Cytometry, Immunocytochemistry
• Isotyp: IgM
• Wirt: Mouse