Mouse anti-NSP-A and NSP-B, clone RNL-2, Purified (Monoclonal)

Clone Number: RNL-2

Immunogen: small cell lung cancer cell line NCI-H82

Concentration: 1 mg/ml

Storage buffer: PBS with 0.09% sodium azide

Additional info: In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).RNL-2 recognizes an epitope located within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.

References: Broers et al. Cancer 1991;67:619-633/Roebroek et al. J Biol Chem 1993;268:13439-47/Senden et al. Int J Cancer Suppl 1994;8:84-8

• Artikelnummer: SANMON2095
• Hersteller: Sanbio / Monosan
• Hersteller Artikelnummer: MON2095
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Reaktivität: Human, Monkey (Primate), Rabbit
• Klonalität: Monoclonal
• Methode: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western Blotting, Flow Cytometry, Immunocytochemistry
• Isotyp: IgG1
• Wirt: Mouse