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Mouse Cot (MAP3K8), His-GST-tags Recombinant
Mouse Cot (MAP3K8), His-GST-tags Recombinant
Artikelnummer
BPS41050
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 30-397
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl2, 0.1 mg/ml BSA) containing 5 µg inactive MEK1 (BPS #40075), 20 µM ATP, and mCOT for 60 minutes at 30°C. The amount of ATP transferred was measured by Kinase-Glo plus (Promega).
Background: COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates IkappaB kinases and induces the nuclear production of NF-κB. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner. Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of Cot results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels.
Description: Mouse Cot kinase (MAP3K8), GenBank Accession No. NM_007746, a.a. 30-397, with N-terminal His-GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. Theoretical MW is 70 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.4 mM KCl, 5 mM glutathione, 134 mM imidazole, 3 mM DTT and 20% glycerol.
Genbank: NM_007746
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST-tags
Uniprot: Q07174
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Jeong, J.H. et al., PLoS One. 2011 Jan 18, 6(1):e16205.
2. Soria-Castro, I. et al. J Biol Chem. 2010 Oct 29,285(44):33805-15.
Encompassing Amino Acids: 30-397
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl2, 0.1 mg/ml BSA) containing 5 µg inactive MEK1 (BPS #40075), 20 µM ATP, and mCOT for 60 minutes at 30°C. The amount of ATP transferred was measured by Kinase-Glo plus (Promega).
Background: COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates IkappaB kinases and induces the nuclear production of NF-κB. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner. Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of Cot results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels.
Description: Mouse Cot kinase (MAP3K8), GenBank Accession No. NM_007746, a.a. 30-397, with N-terminal His-GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. Theoretical MW is 70 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.4 mM KCl, 5 mM glutathione, 134 mM imidazole, 3 mM DTT and 20% glycerol.
Genbank: NM_007746
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST-tags
Uniprot: Q07174
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Jeong, J.H. et al., PLoS One. 2011 Jan 18, 6(1):e16205.
2. Soria-Castro, I. et al. J Biol Chem. 2010 Oct 29,285(44):33805-15.
| Artikelnummer | BPS41050 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 41050 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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Gernot Ensinger, M.Sc.
Laurent Haze
