Mouse CTLA4(Biotin):B7-1 Inhibitor Screening Assay Kit

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Background: The activation of naÍve T cells requires two signals, the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cell surface. Conversely, binding of CTLA4 to B7-1 on the T-cell surface results in an inhibitory signal and prevents T-cell activation. CTLA4:B7-1 interaction is an important drug target for the regulation of the host's response to cancer.

Description: The Mouse CTLA4[biotin]:B7-1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of mouse CTLA4:B7-1 signaling. This kit comes in a convenient 96-well format, with biotin-labeled mouse CTLA4, purified mouse B7-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled mouse CTLA4 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, B7-1 is coated on a 96-well plate. Next, CTLA4[biotin] is incubated with B7-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Storage Stability: One year from date of receipt when stored as directed.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Ohtani, H., et al., Lab Invest. 1997; 77(3): 231-241.2. Rovert, C., et al., N. Engl. J. Med. 2011; 364: 2517-25262.

• Artikelnummer: BPS79515
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79515
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK