Mouse EGFR, FLAG-Tag Recombinant

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Encompassing Amino Acids: 670-1210

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed in a Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 µM ATP. The reaction to place at 30°C for 40 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Description: Mouse EGFR (GenBank Accession No. NM_207655/NP_997538), a.a. 670-1210 with a C-terminal FLAG-tag, MW = 62 kDa, expressed in SF9 cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol

Genbank: NM_207655

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal FLAG-tag

Uniprot: Q01279

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Mackenzie, J. et al., Mol Immunol. 2011 Jul,48(12-13):1578-85
2. Sadri, K. et al., Nucl Med Commun. 2011 Jul,32(7):563-9.

• Artikelnummer: BPS40195
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40195
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect