Mouse IDO1 Inhibitor Screening Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Useful for the study of mouse IDO1 enzymology, screening inhibitors and selectivity profiling.

Background: L-tryptophan (L-Trp) is an essential amino acid necessary for proteinsynthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established asa key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains animmunosuppressive microenvironment by starving immune cells of L-Trp and releasingdegradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulatedin many tumors, providing cancer cells with an avenue for immune evasion.

Contraindications: DMSO >0.5%, strong acids or bases, ionic detergents, high salt

Description: The Mouse IDO1 Inhibitor Screening Assay Kit is designed to measure inhibition of the murine IDO1 enzyme. This kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at l=320-325 nm.

Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Supplied As: The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Koblish, H.K., et al., Mol Cancer Ther, 2010; 9(2): 489-98
2. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
3. Seegers, N., et al. J Biomol Screen. 2014; 19(9):1266-74.

• Artikelnummer: BPS72041
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 72041
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK