Mouse ITK Kinase Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Background: ITK (IL-2-inducible T cell kinase), is a cytosolic tyrosine kinase expressed in T cells. ITK contains two protein binding domains, SH2 and SH3, which allows the kinase to be recruited by tyrosine-phosphorylated proteins, notably upon activation of the T Cell Receptor (TCR). It plays an important role in TCR signal transduction pathways and ultimately leads to the activation of the transcription factor NFAT (nuclear factor of activated T cells), cytokine secretion, and T cell differentiation and expansion.

Contraindications: The final concentration of DMSO in the assay should not exceed 1%.

Description: The Mouse ITK Kinase Assay Kit is designed to measure recombinant murine ITK kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent. 

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant Mouse ITK kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Uniprot: Q03526

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS78520
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78520
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK