Mouse JARID1B (KDM5B, PLU-1), FLAG-tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 2-751

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: Histone demethylase that demethylates Lys-4 of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 Lys-9 or H3 Lys-27. Demethylates trimethylated, dimethylated and monomethylated H3 Lys-4. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.

Description: Mouse JARID1B, also known as PLU-1 and KDM5B (GenBank Accession No. NM_152895), a.a. 2-751 with N-terminal FLAG-tag, MW = 86 kDa, expressed in Sf9 cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ?g/ml FLAG peptide, 10% glycerol, and 3 mM DTT

Genbank: NM_152895

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-tag

Uniprot: Q80Y84

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Yamane K, et al. (2007). Mol. Cell 25 (6): 801-12.
2. Barrett A, et al. (2007). Int. J.Cancer 121 (2): 265-75.Application Reference:
An high-throughput in vivo screening system to select H3K4-specific histone demethylase inhibitors (2014)

• Artikelnummer: BPS50122
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50122
• Green Labware: Nein
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Wirt: Insect