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Encompassing Amino Acids: 25-198
Applications: Useful for studying protein binding and for screening small molecules and antibodies.
Description: Mouse sonic hedgehog (mShh), also knownas HHG-1. GenBank Accession No.NM_009170, a.a. 25-198 with a C-terminalHis-tag, expressed in an E. coli cellexpression system, MW = 20.5 kDa.
Endotoxin Level: <1 EU/µg
Format: Aqueous buffer solution
Formulation: 10 mM Na phosphate pH 7.4, 138 mM NaCl, 2.75 mM KCl.
Genbank: NM_009170
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: Q62226
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tran, P.V., et al., Nat. Genet. 2008; 40:403-410.
2. Mukhopadhyay, S., et al., Cell 2012; 152(1-2): 210-223.