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Mouse Sphingosine kinase 1a, His-tag Recombinant
Mouse Sphingosine kinase 1a, His-tag Recombinant
Artikelnummer
BPS40613
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 4 µM D-erythrosphingosine, and 0.1-0.5 µg/ml sphingosine kinase 1 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega Corporation, Madison, WI).
Description: Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His-tag, MW=47.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Genbank: AF415213
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Unit Definition: A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004)
2. Taha, TA et al., FASEB J. 20(3):482-4 (2005)
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 4 µM D-erythrosphingosine, and 0.1-0.5 µg/ml sphingosine kinase 1 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega Corporation, Madison, WI).
Description: Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His-tag, MW=47.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Genbank: AF415213
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Unit Definition: A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004)
2. Taha, TA et al., FASEB J. 20(3):482-4 (2005)
| Artikelnummer | BPS40613 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40613 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) |
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| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
