Mouse Transferrin

Application Note: Mouse transferrin is supplied in 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH: 7.6. Mouse Transferrin has been tested by SDS-PAGE and is a suitable protein for use as a control reagent in SDS, Western Blotting, and ELISA experiments. For blotting, the expected molecular weight of Mouse Transferrin is approximately 80kDa. Mouse Transferrin, when unopened, is stable at 4° C, but for extended use should be aliquoted and stored at -20° C in its undiluted form. If dilutions of Mouse Transferrin are required, they should be prepared just prior to use and not retained.

Concentration Value: 6.0 mg/ml

ELISA Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Mouse Transferrin was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis against the buffer stated above.  Mouse Transferrin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Transferrin and anti-Mouse Serum.

Background: Mouse Transferrin is a blood plasma glycoprotein which binds iron and regulates the amount of available iron in circulation. Mouse Transferrin has a high affinity for iron, but this binding is reversible. Mouse Transferrin has a molecular weight of approximately 80kDa. Mouse Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive. This product is holo-transferrin and is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC010-0134
• Hersteller: Rockland
• Hersteller Artikelnummer: 010-0134
• Verpackungseinheit: 5 mg
• Mengeneinheit: STK
• Methode: SDS-PAGE
• Isotyp: Transferrin
• Human Gene ID: 2204
• Konjugat: Unconjugated