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MTAP, GST-tag Recombinant
MTAP, GST-tag Recombinant
Artikelnummer
BPS50305
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: MTAP activity wasmeasured using a xanthine oxidase-coupledmethod. MTA cleavage by MTAP produceadenine, which is then converted to 2,8-dihydroxyadenine by xanthine oxidase,leading to an increase in absorbance at 305nm. 50 µl reaction mixtures contained MTAP,50 mM KHsub>2PO4, pH 7.4, 200 µM MTA, 0.02units Xanthine Oxidase, and 1 mM DTT.Activity was measured at 37°C by continuousmonitoring the absorbance at 305 nm for 30min.
Description: Human MTAP (methylthioadenosine phosphorylase), GenBank Accession No. NM_002451, a.a. 2-end, with N-terminal GST-tag, MW=57 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Genbank: NM_002451
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q13126
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Fernandez-Irigoyen, J. et al., Biochem. J. 411 (2), 457-465 (2008)
2. Hellerbrand, C. et al., Carcinogenesis 27 (1), 64-72 (2006)
Encompassing Amino Acids: 2-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: MTAP activity wasmeasured using a xanthine oxidase-coupledmethod. MTA cleavage by MTAP produceadenine, which is then converted to 2,8-dihydroxyadenine by xanthine oxidase,leading to an increase in absorbance at 305nm. 50 µl reaction mixtures contained MTAP,50 mM KHsub>2PO4, pH 7.4, 200 µM MTA, 0.02units Xanthine Oxidase, and 1 mM DTT.Activity was measured at 37°C by continuousmonitoring the absorbance at 305 nm for 30min.
Description: Human MTAP (methylthioadenosine phosphorylase), GenBank Accession No. NM_002451, a.a. 2-end, with N-terminal GST-tag, MW=57 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Genbank: NM_002451
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q13126
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Fernandez-Irigoyen, J. et al., Biochem. J. 411 (2), 457-465 (2008)
2. Hellerbrand, C. et al., Carcinogenesis 27 (1), 64-72 (2006)
| Artikelnummer | BPS50305 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 50305 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
