MYLK3, GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: MYLK3 (or myosin light chain kinase 3) plays a key role in rate of cross-bridge recruitment in cardiac myocytes. MYLK3 is upregulated in failing human myocardia and expression of MYLK3 correlates with the pulmonary arterial pressure of patients with heart failure (1). Knockdown of MYLK3 with small interfering RNAs in cultured rat cardiomyocytes decreases Myl2 phosphorylation and impairs epinephrine-induced activation of sarcomere reassembly (2). Recombinant murine MYLK3 shows specificity for Myl2 (Mlc2v) and the phosphorylation is calcium- and calmodulin-dependent.

Description: Recombinant human MYLK3, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: BC109097

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q32MK0

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Chan, J. Y. et al:Identification of cardiac-specific myosin light chain kinase. Circ. Res.2008; 102: 571-580.
2. Seguchi, O. et al: A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart. J. Clin. Invest. 2007;117: 2812-2824.

• Artikelnummer: BPS40136
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40136
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect