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NEDD8 E1, His-tag Recombinant
NEDD8 E1, His-tag Recombinant
Artikelnummer
BPS81087
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Applications: Useful for NEDD8 conjugation reactions and NEDDylation pathway studies
Background: The NEDD8 activating E1 enzyme is a heterodimer composed of APPBP1 and UBA3 subunits. The APPBP1/UBA3 enzyme has homology to the N- and C-terminal halves of the ubiquitin E1 enzyme, respectively. The UBA3 subunit contains the catalytic center and activates NEDD8 in an ATP-dependent reaction by forming a high-energy thiolester intermediate. The activated NEDD8 is subsequently transferred to the UbcH12 E2 enzyme, and is then conjugated to specific substrates in the presence of the appropriate E3 ligases.
Biological Activity: 1-100 nM is used for the in vitro conjugation
Description: Human NEDD8 E1 recombinant protein complex, with His-tagged APPBP1 and untagged Uba3, co-expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 50 mM Tris Buffer, pH 8.0, 150 mM NaCl, 10% glycerol
Purity: ≥80%
Storage Stability: Store at or below -80°C. Stable as supplied for up to 1 year when stored dessicated at -80°C.
Tags: His-tag (APPBP1), no tag (Uba3)
Uniprot: Q8TBC4
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
Applications: Useful for NEDD8 conjugation reactions and NEDDylation pathway studies
Background: The NEDD8 activating E1 enzyme is a heterodimer composed of APPBP1 and UBA3 subunits. The APPBP1/UBA3 enzyme has homology to the N- and C-terminal halves of the ubiquitin E1 enzyme, respectively. The UBA3 subunit contains the catalytic center and activates NEDD8 in an ATP-dependent reaction by forming a high-energy thiolester intermediate. The activated NEDD8 is subsequently transferred to the UbcH12 E2 enzyme, and is then conjugated to specific substrates in the presence of the appropriate E3 ligases.
Biological Activity: 1-100 nM is used for the in vitro conjugation
Description: Human NEDD8 E1 recombinant protein complex, with His-tagged APPBP1 and untagged Uba3, co-expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 50 mM Tris Buffer, pH 8.0, 150 mM NaCl, 10% glycerol
Purity: ≥80%
Storage Stability: Store at or below -80°C. Stable as supplied for up to 1 year when stored dessicated at -80°C.
Tags: His-tag (APPBP1), no tag (Uba3)
Uniprot: Q8TBC4
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS81087 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 81087 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) |
|
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
