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NEK6, His-tag Recombinant
NEK6, His-tag Recombinant
Artikelnummer
BPS40014
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 1-313
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.1 mM ATP, 4 µM NEK6 substrate, and 0.4 µg/ml NEK6.
Description: Human NEK6 (GenBank Accession No. NM_014397), a.a. 1-313 with N-terminal His-tag, MW=40.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Genbank: NM_014397
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q9HC98
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol NEK6 substrate (FLAKSFGSPNRAYKK) per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yin, M.J. et al., J. Biol. Chem. 278 (52), 52454-52460 (2003)
2. Belham, C. et al., J. Biol. Chem. 278 (37), 34897-34909 (2003)
Encompassing Amino Acids: 1-313
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.1 mM ATP, 4 µM NEK6 substrate, and 0.4 µg/ml NEK6.
Description: Human NEK6 (GenBank Accession No. NM_014397), a.a. 1-313 with N-terminal His-tag, MW=40.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Genbank: NM_014397
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q9HC98
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol NEK6 substrate (FLAKSFGSPNRAYKK) per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yin, M.J. et al., J. Biol. Chem. 278 (52), 52454-52460 (2003)
2. Belham, C. et al., J. Biol. Chem. 278 (37), 34897-34909 (2003)
| Artikelnummer | BPS40014 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 40014 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) |
|
| MSDS (PDF) |
|
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