NEK7, GST-tag Recombinant

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Encompassing Amino Acids: 1-302(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was performed in a 25 µl reaction containing 40 mM Tris, pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA, 100 µM ATP, 0.2 mg/ml Casein and NEK7 for 50 min at 30°C. NEK7 activity was measured using ADP-Glo reagents (Promega).

Description: Human NEK7, also known as NIMA Related Kinase 7 (GenBank Accession No. NM_133494), full length, a.a. 1-302 (end), with N-terminal GST-tag, MW=61.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT and 20% glycerol

Genbank: NM_133494

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q8TDX7

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Kandli, M., et al. Genomics 68: 187-196 (2000).
2. Belham, C., et al. Curr. Biol. 15: 1155-1167 (2001).

• Artikelnummer: BPS40141
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40141
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect