Neutrophil Elastase Inhibitor Screening Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Application: Screen small molecule inhibitors in high throughput screening (HTS) applications.

Background: Elastase is a serine protease from the chymotrypsin family involved in the degradation of elastin and some viral proteins. Elastase exists as pancreatic and neutrophil elastase, which differ in their specificity. Neutrophil elastase is also known as ELA2 or polymorphonuclear leukocyte elastase and is present in azurophilic granules. Upon neutrophil activation, degranulation occurs and serine proteases, such as elastase, are released into the extracellular space to degrade the matrix proteins of pathogens. Alterations in the levels of elastase, or dysfunction of its endogenous inhibitors, result in alpha-1 antitrypsin deficiency (A1AD), emphysema, cyclic neutropenia, abdominal aortic aneurysms (AAA) and chronic obstructive pulmonary disease (COPD), arthritis and psoriasis. The use of neutrophil elastase inhibitors, such as elafin, and the development of new inhibitors with fewer side effects may benefit patients suffering from elastase-related diseases.

Description: The Neutrophil Elastase Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of elastase for screening and profiling applications. The elastase assay kit comes in a convenient 96-well or 384-well format and contains enough purified elastase, fluorochrome-conjugated substrate, and PR-02 buffer for 100 or 400 reactions. This kit also includes Sivelestat as control inhibitor.PR Substrate 3 is a fluorogenic peptide substrate for neutrophil elastase. In the conjugated form the energy emitted from the fluorochrome AMP is quenched. Proteolysis releases AMP and fluorescence is emitted. The increase in fluorescence is directly proportional to elastase activity.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Uniprot: P08246

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Jakimiuk K, et al., 2021 J Enzyme Inhib Med Chem 36(1): 1016-1028.

• Artikelnummer: BPS82090-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82090-1
• Verpackungseinheit: 96 reactions
• Mengeneinheit: PAK