NFAT Reporter (Luciferase) - THP-1 Cell Line

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Applications: Monitor NFAT signaling pathway activity.
Screen for compound activity on the NFAT signaling pathway.

Description: The NFAT reporter (Luciferase)-THP-1 cell line is designed for monitoring the NFAT (nuclear factor of activated T-cells) signaling pathway in THP-1 cells by measuring luciferase activity. It contains a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter. Upon activation by NFAT activators such as Ionomycin, endogenous NFAT transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

Formulation: Thaw Medium 2 (BPS Bioscience, #60184): RPMI1640 medium (Life Technologies, #A10491-01) supplemented with 10% FBS (Life Technologies, #26140-079), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).Growth Medium 2M (BPS Bioscience, #78181): Thaw Medium 2 (BPS Bioscience, #60184) plus 1 µg/ml of Puromycin (Invivogen, #ant-pr-1).Assay Medium: Thaw Medium 2 (BPS Bioscience, #60184)

Host Cell Line: THP-1

Mycoplasma Testing: The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.

Storage Stability: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Supplied As: 2 vials: Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

References: 1. Clipstone NA, Crabtree GR. Nature. 1992 Jun 25;357(6380):695-7.
2. Lyakh, L., et al. Mol Cell Biol. 1997 May;17(5):2475-84.

• Artikelnummer: BPS78320
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78320
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Human