NMNAT1, His-Tag Recombinant

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Encompassing Amino Acids: 2-279

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: The 50 µL reaction mixture contained 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2, 0.1 mM NMN, 0.1 mM ATP, and various amounts of NMNAT. The reaction was incubated at 37°C for 30 min, and the amount of ATP consumed was calculated using Kinase-Glo® Plus reagent (Promega).

Description: Human nicotinamide nucleotide adenylyltransferase 1, also known as NMNAT1, (GenBank Accession No. NM_022787), a.a. 2-279 with N-terminal His-tag, MW = 32.8 kDa, expressed in an E. coli cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 256 mM imidazole and 20% glycerol

Genbank: NM_022787

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: Q9HAN9

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Kaplan, J., et al., Med Sci (Paris). 2013; 29(1):26-27
2. Chiang, P.W., et al., Nat Genet. 2012; 44(9):972-974

• Artikelnummer: BPS71090
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 71090
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK