NMNAT2, His-Tag Recombinant

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Encompassing Amino Acids: 2-307

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: T50 mM Tris/HCl, pH 7.4, 5 mM MgCl2, 0.05% Tween-20, 0.1 mM NMN, 0.1mM ATP, and NMNAT2 (3.2 - 200ng) in a final volume of 50 µL. The reaction was incubated for 30 min at 37ºC. The amount of ATP consumed was calculated using Kinase-Glo plus reagent (Promega).

Description: Human nicotinamide nucleotide adenylyltransferase 2, also known as NMNAT2, (GenBank Accession No. NM_015039), a.a. 2-307 (end) with N-terminal His-tag, MW = 33 kDa, expressed in an E. coli cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3mM DTT, 375 mM imidazole

Genbank: NM_015039

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: Q9BZQ4

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Yan, T., et al., NCI. 2010; 56(1): 101-106.Ljungberg, M. et al., Human Molecular Genetics. 2011; 21(2): 251-267.

• Artikelnummer: BPS100198
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100198
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK