Notch1 Antibody, Clone N253/32: Biotin

Mouse Anti-Mouse Notch1 Monoclonal IgG1
Artikelnummer
STRSMC-430D-BI
Verpackungseinheit
100 µg
Hersteller
Stressmarq Biosciences

Verfügbarkeit: wird geladen...
Preis wird geladen...
Target: Notch1

Conjugate: Biotin

Product Type: Monoclonal

Clone Number: N253/32 (Formerly sold as S253-32)

Immunogen: Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1

Swiss-Prot: Q01705

Purification: Protein G Purified

Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated

Concentration: 1 mg/ml

Specificity: Detects >270kDa. Does not cross-react with Notch 2 or Notch3.

Cellular Localization: Cell Membrane,Nucleus

Scientific Background: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

References: 1. Swiatek P.J., et al. (1994) Genes Dev. 8: 707-719.2. Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company.3. Lindsell C.E., et al. (1995) Cell 80: 909-917.4. Uyttendaele H., et al. (1996) Development 122: 2251-2259.5. Girard L., et al. (1996) Genes Dev. 10: 1930-1944.

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Mehr Informationen
Artikelnummer STRSMC-430D-BI
Hersteller Stressmarq Biosciences
Hersteller Artikelnummer SMC-430D-BI
Green Labware Nein
Verpackungseinheit 100 µg
Mengeneinheit STK
Reaktivität Human, Mouse (Murine), Rat (Rattus)
Klonalität Monoclonal
Methode Immunofluorescence, Western Blotting, Immunocytochemistry
Isotyp IgG1
Human Gene ID 18128
Wirt Mouse
Produktinformation (PDF) Download
MSDS (PDF) Download