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NUAK1, GST-Tag
NUAK1, GST-Tag
Artikelnummer
BPS101475
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: Full Length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Kinase activity was measured using a CHKtide peptide substrate (KKKVSRSGLYRSPSMPENLNRPR) diluted in distilled water to a final concentration of 1 mg/ml. Increasing amounts of kinase were mixed with CHKtide peptide substrate with a final concentration of 200 µg/ml in a buffer containing 5 mM MOPS, pH 7.2, 2.5 mM β-glycerol-phosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA and 0.05 mM fresh DTT to a final volume of 20 µl. The reaction was initiated by addition of 5 µl of [33P]-ATP diluted in kinase buffer: 6 ml kinase buffer containing 1 mCi [33P]-ATP, 0.25 mM ATP, 25 mM MOPS, pH 7.2, 12.5 mM β-glycerophosphate, 25 mM MgCl2, 5 mM EGTA, 2 mM EDTA, and 0.25 mM fresh DTT. After incubating for 30°C for 15 minutes, the reaction was terminated by spotting 20 µl of the mixture onto phosphocellulose paper strips that were fixed in 1% phosphoric acid and washed three times. Radioactivity was determined using a scintillation counter. The blank was determined from a "no substrate" sample.
Description: Recombinant human NUAK1 (SNF1-like kinase 1) full length protein containing an N-terminal GST-tag. This recombinant protein was affinity purified and is kinase active.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_014840
Purity: ≥80%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O60285
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
Encompassing Amino Acids: Full Length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Kinase activity was measured using a CHKtide peptide substrate (KKKVSRSGLYRSPSMPENLNRPR) diluted in distilled water to a final concentration of 1 mg/ml. Increasing amounts of kinase were mixed with CHKtide peptide substrate with a final concentration of 200 µg/ml in a buffer containing 5 mM MOPS, pH 7.2, 2.5 mM β-glycerol-phosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA and 0.05 mM fresh DTT to a final volume of 20 µl. The reaction was initiated by addition of 5 µl of [33P]-ATP diluted in kinase buffer: 6 ml kinase buffer containing 1 mCi [33P]-ATP, 0.25 mM ATP, 25 mM MOPS, pH 7.2, 12.5 mM β-glycerophosphate, 25 mM MgCl2, 5 mM EGTA, 2 mM EDTA, and 0.25 mM fresh DTT. After incubating for 30°C for 15 minutes, the reaction was terminated by spotting 20 µl of the mixture onto phosphocellulose paper strips that were fixed in 1% phosphoric acid and washed three times. Radioactivity was determined using a scintillation counter. The blank was determined from a "no substrate" sample.
Description: Recombinant human NUAK1 (SNF1-like kinase 1) full length protein containing an N-terminal GST-tag. This recombinant protein was affinity purified and is kinase active.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_014840
Purity: ≥80%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O60285
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS101475 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 101475 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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