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NUAK2, GST-Tag
NUAK2, GST-Tag
Artikelnummer
BPS101476
Verpackungseinheit
10 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: Full Length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Kinase activity was measured using ADP-Glo™ Kinase Assay Kit (Promega; Cat# V9101) which quantifies the amount of ADP produced by the NUAK2 reaction. The ADP-Glo™ Reagent is added to terminate the reaction and quench the remaining ATP. The Kinase Detection Reagent is then added to convert ADP to ATP and to measure the newly converted ATP using a luciferase/luciferin reaction.
Assay: Kinase activity was measured using substrate CHKtide (KKKVSRSGLYRSPSMPENLNRPR) (diluted in distilled water to a final concentration of 1 mg/ml. Increasing amounts of NUAK2 kinase were incubated with a final concentration of 0.2 mg/ml substrate in 40 mM Tris-HCl, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, and 50 µM fresh DTT. The reaction was initiated by adding a final concentration of 25 µM ATP and incubating for 40 minutes at room temperature. The reaction was terminated by the addition of 5 µl of ADP-Glo™ Reagent and a subsequent incubation at room temperature for 40 minutes. Luminescence was measured by the addition of 10 µl Kinase Detection Reagent followed by incubation at room temperature for an additional 30 minutes. The blank was determined from a "no kinase" sample.
Description: Recombinant human NUAK2 (SNF1-like kinase 2) full length protein containing an N-terminal GST-tag. This recombinant protein was affinity purified and is kinase active.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_030952
Purity: ≥70%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9H093
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
Encompassing Amino Acids: Full Length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Kinase activity was measured using ADP-Glo™ Kinase Assay Kit (Promega; Cat# V9101) which quantifies the amount of ADP produced by the NUAK2 reaction. The ADP-Glo™ Reagent is added to terminate the reaction and quench the remaining ATP. The Kinase Detection Reagent is then added to convert ADP to ATP and to measure the newly converted ATP using a luciferase/luciferin reaction.
Assay: Kinase activity was measured using substrate CHKtide (KKKVSRSGLYRSPSMPENLNRPR) (diluted in distilled water to a final concentration of 1 mg/ml. Increasing amounts of NUAK2 kinase were incubated with a final concentration of 0.2 mg/ml substrate in 40 mM Tris-HCl, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, and 50 µM fresh DTT. The reaction was initiated by adding a final concentration of 25 µM ATP and incubating for 40 minutes at room temperature. The reaction was terminated by the addition of 5 µl of ADP-Glo™ Reagent and a subsequent incubation at room temperature for 40 minutes. Luminescence was measured by the addition of 10 µl Kinase Detection Reagent followed by incubation at room temperature for an additional 30 minutes. The blank was determined from a "no kinase" sample.
Description: Recombinant human NUAK2 (SNF1-like kinase 2) full length protein containing an N-terminal GST-tag. This recombinant protein was affinity purified and is kinase active.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_030952
Purity: ≥70%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9H093
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS101476 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 101476 |
| Green Labware | Nein |
| Verpackungseinheit | 10 µg |
| Mengeneinheit | STK |
| Wirt | Insect |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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