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Applications: Human otubain 2 is inactive in vitro against peptide and isopeptide-linked substrates 26, but cleaves Ub-AMC .
Background: Otubain 2 is a member of the OTU (ovarian tumor) superfamily of ubiquitin isopeptidases. It was originally identified as a protein capable of binding ubiquitin aldehyde. Researchers have shown that it is able to cleave tetra-ubiquitin chains in vitro only after removal of a 5 residue C-terminal extension.
Description: Human Otub2 recombinant protein (Genbank Accession No. Q96DC9), with His-tag, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM DTT
Genbank: Q96DC9
Purity: ≥95% by SDS-PAGE
Storage Stability: Store at or below -80°C. Stable as supplied for up to 1 year when stored dessicated at -80°C.
Tags: His-tag
Uniprot: Q96DC9
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Nanao, MH., et al. EMBO Rep. 5, 783-8 (2004).
2. Balakirev, M. et al. EMBO Rep. 2003. 4(5): p517-522.
3. Li, S., et al. J Biol Chem. 285, 4291-7 (2010).