p120GAP, His-Tag Recombinant

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Applications: Useful for the study of RAS GTPase activation.

Assay Conditions: GTPase activity of KRAS(WT, 50ng) and KRAS(G12C, 100ng) were tested in the presence of various concentration of p120GAP (0 ng - 200 ng) and 2 µM GTP by using GTPase-Glo reagents (Promega). Reactions were done at room temperature for 80 min.

Description: Human Ras GTPase-activating protein 1, also known as p120GAP, RASA1, GAP and RASA, GenBank Accession No. NM_002890, a.a. 714-1047(end) with an N-terminal His-Tag, expressed in an E. coli cell expression system. MW=39 kDa.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol.

Genbank: NM_002890

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: N-terminal His-Tag

Uniprot: P20936

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Scheffzek, K., et al., Nature. 1996; 384: 591-596
2. Torit, M. et al., JBC. 1992; 267: 8293-8298

• Artikelnummer: BPS100518-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100518-1
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK